hela 229 cell monolayers Search Results


96
ATCC cycloheximide treated hela 229 cell monolayers
Cycloheximide Treated Hela 229 Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC hela 229 cell monolayers
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Hela 229 Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hela 229 cell monolayers/product/ATCC
Average 94 stars, based on 1 article reviews
hela 229 cell monolayers - by Bioz Stars, 2026-03
94/100 stars
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98
ATCC hela 229 epithelial cell monolayers
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Hela 229 Epithelial Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hela 229 epithelial cell monolayers/product/ATCC
Average 98 stars, based on 1 article reviews
hela 229 epithelial cell monolayers - by Bioz Stars, 2026-03
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Image Search Results


In situ identification of C. pneumoniae in HeLa 229 cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.

Journal:

Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization

doi: 10.1128/AEM.68.8.4081-4089.2002

Figure Lengend Snippet: In situ identification of C. pneumoniae in HeLa 229 cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.

Article Snippet: Chlamydial strains were maintained on cycloheximide-treated HeLa 229 cell monolayers (CCL2.1; American Type Culture Collection) in six-well culture plates by standard procedures.

Techniques: In Situ, Labeling, Fluorescence

Coinfection of HeLa 229 cells with C. trachomatis and C. pneumoniae. The simultaneous application of FLUOS-labeled probe Ct-623 specific for C. trachomatis (A) (green) and Cy5-labeled probe Cpn-214 specific for C. pneumoniae (B) (blue) allows the differentiation between the two chlamydia species. The simultaneous use of the Cy3-labeled eukaryotic probe EUK516 (C) (red) clearly demonstrated that a single HeLa 229 cell can be infected with both chlamydiae (D). Bar, 10 μm.

Journal:

Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization

doi: 10.1128/AEM.68.8.4081-4089.2002

Figure Lengend Snippet: Coinfection of HeLa 229 cells with C. trachomatis and C. pneumoniae. The simultaneous application of FLUOS-labeled probe Ct-623 specific for C. trachomatis (A) (green) and Cy5-labeled probe Cpn-214 specific for C. pneumoniae (B) (blue) allows the differentiation between the two chlamydia species. The simultaneous use of the Cy3-labeled eukaryotic probe EUK516 (C) (red) clearly demonstrated that a single HeLa 229 cell can be infected with both chlamydiae (D). Bar, 10 μm.

Article Snippet: Chlamydial strains were maintained on cycloheximide-treated HeLa 229 cell monolayers (CCL2.1; American Type Culture Collection) in six-well culture plates by standard procedures.

Techniques: Labeling, Infection

Combination of FISH and DFA. The performance of DFA subsequent to FISH allows the identification of chlamydiae (by the rRNA-targeted oligonucleotide probes) simultaneously with the detection of chlamydial antigen (by antichlamydia antibodies). (A) Fluorescent signal derived from DFA using fluorescein-labeled antichlamydia-LPS antibodies. (B) Fluorescent signal derived from FISH with C. pneumoniae-specific probe Cpn-214 labeled with Cy3. (C) Fluorescent signal derived from DAPI staining of chlamydiae and nuclei of the HeLa 229 host cells. (D) Composite image. Bar, 10 μm.

Journal:

Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization

doi: 10.1128/AEM.68.8.4081-4089.2002

Figure Lengend Snippet: Combination of FISH and DFA. The performance of DFA subsequent to FISH allows the identification of chlamydiae (by the rRNA-targeted oligonucleotide probes) simultaneously with the detection of chlamydial antigen (by antichlamydia antibodies). (A) Fluorescent signal derived from DFA using fluorescein-labeled antichlamydia-LPS antibodies. (B) Fluorescent signal derived from FISH with C. pneumoniae-specific probe Cpn-214 labeled with Cy3. (C) Fluorescent signal derived from DAPI staining of chlamydiae and nuclei of the HeLa 229 host cells. (D) Composite image. Bar, 10 μm.

Article Snippet: Chlamydial strains were maintained on cycloheximide-treated HeLa 229 cell monolayers (CCL2.1; American Type Culture Collection) in six-well culture plates by standard procedures.

Techniques: Derivative Assay, Labeling, Staining